Nils Helge Schebb

Sanofi-Aventis-Preis (Vortrag) 2014 für Nils Helge Schebb

Metabolic Activation of the Antibacterial Triclocarban by Cytochrome P450 1A1 Yielding Glutathione and Protein Adducts

Triclocarban (TCC) is a common antimicrobial preservative in personal care products, particularly in bar soaps. TCC accumulates in the aquatic environment and has biological effects on mammals. For example, it is a potent inhibitor of the soluble epoxide hydrolase, an enzyme of the arachidonic cascade thus raising the possibility of detrimental effects upon human exposure. Bathing with TCC-containing soaps results in deposition of TCC on human skin and a small portion of this amount traverses the epidermal barrier. The absorbed TCC is extensively metabolized by Cytochrome P450 monooxygenases (CYPs). In order to investigate whether the metabolic activation of TCC might occur in skin incubations with spontaneously immortalized human epidermal keratinocytes (SIK), a minimally deviated epidermal culture model, were carried out with and without pre-induction with the Ah-receptor agonist TCCD. Upon treatment, TCC was rapidly absorbed from the medium (2 µM) reaching a level of 2.04 ± 0.27 nmol/mg protein. At baseline a small but significant portion of the absorbed TCC was oxidatively metabolized by SIK while pre-incubation of SIK with 10 nM TCCD dramatically augmented metabolism of TCC. In order to test whether reactive metabolites were formed, proteins recovered from SIK incubations with 14C-TCC were isolated, and the levels of bound radioactivity were measured by accelerator mass spectrometry. Up to 23 ± 2 pmol/mg TCC equivalents were covalently bound to cellular protein after 24 h of incubation in cells induced with TCDD [1]. Consistent with these findings, TCC-glutathione adduct peaks were detected in liquid chromatography (LC) analysis and scintillation counting following incubation of 14C-TCC with CYP1A1, the major TCCD-inducible CYP-isoform. The main GSH-adduct was thoroughly characterized by means of liquid chromatography with high resolution mass spectrometry [2]. These results suggest that TCC is activated via oxidative dehalogenation yielding a reactive para quinone imine. Overall, the reactive metabolites were only found in low levels which does not necessarily translate to potential adverse health effects following the use of TCC in personal care products. However, it is a surprise that reactive metabolite formation of this major ingredient in personal care products has been overlooked for more than 50 years of usage.

[1] N.H. Schebb, B. Buchholz, B.D. Hammock and R.H. Rice (2012) Metabolism of the Antibacterial Triclocarban by Human Epidermal Keratinocytes to Yield Protein Adducts. J Biochem Mol Toxic, 26 (6), 230-234.

[2] N. H. Schebb, J. B. Muvvala, D. Morin, A. R. Buckpitt, B. D. Hammock and R. H. Rice (2014) Metabolic activation of the antibacterial agent, triclocarban, by cytochrome P450 1A1 yielding glutathione adducts. . Drug Metab Dispos, doi:10.1124/dmd.114.058206

Zusammenfassung

Stellenangebote

Interessante Stellenangebote finden Sie unter folgendem Link: www.toxikologie.de/...

Sonderheft "Human Biomonitoring 2016" erschienen

Das Sonderheft "Human Biomonitoring 2016", Volume 220/2 Part A der Zeitschrift International Journal of Hygiene and Environmental Health ist erschienen. Insgesamt 34 Beiträge stellen den aktuellen Stand des weltweiten Human-Biomonitorings (HBM) dar.

Das Sonderheft wurde im Nachgang zur 2. Internationalen Human-Biomonitoring Konferenz, Berlin 2016, zusammengestellt, die unter dem Motto "Wissenschaft und Politik für eine gesunde Zukunft" gemeinsam vom Umweltbundesamt und dem Bundesministerium für Umwelt, Naturschutz, Bau und Reaktorsicherheit durchgeführt wurde.

Alle Artikel sind ein Jahr im Open-Access online frei verfügbar: www.sciencedirect.com/...

Poster der GT auf der SOT-Tagung 2017

Society of Toxicology 56th Annual Meeting and ToxExpo, March 12–16, 2017, in Baltimore, Maryland
Poster

Expertinnen und Experten zum Schutz von Versuchstieren gesucht

Der Nationale Ausschuss Tierschutzgesetz (TierSchG) des BfR baut einen ehrenamtlich arbeitenden Expertenpool auf.

Weiterführende Informationen finden sich unter:
www.dgpt-online.de/...

Poster der GT auf der SOT-Tagung 2016

Society of Toxicology 55th Annual Meeting and ToxExpo, March 13–17, 2016, in New Orleans
Poster

Wissenschafltiche Ausarbeitung

Arsen in Lebensmitteln und in Trinkwasser
Positionspapier

Pressemitteilung

Toxikologen warnen: Gesundheits- und Umweltschutz in Gefahr; Fachgesellschaft beklagt Mangel an Ausbildungsplätzen
PDToxGes2015.pdf

Wissenschafltiche Ausarbeitung

Toxikologie in Deutschland 2015
Positionspapier

Positionspapier des Arbeitskreises zum Humanbiomonitoring


Humanbiomonitoring.pdf